Fig. 3. Oxidative stress is not affecting mitochondrial proteins at OB. (A) Mitochondria were isolated from hearts and analyzed by (A) Aconitase activity, (B) protein carbonylation (C), thiol groups and (D) ANT oxidation. The data are presented as mean ± SEM. A t-test was performed to obtain the statistical analysis. Green bars indicate CTL groups and red bars 12-mo OB groups (n=6 animals for group). CS: citrate synthase.